Antiviral activity of a serine protease from the digestive juice of Bombyx mori larvae against nucleopolyhedrovirus
نویسندگان
چکیده
منابع مشابه
A lipase isolated from the silkworm Bombyx mori shows antiviral activity against nucleopolyhedrovirus.
A protein showing strong antiviral activity against Bombyx mori nucleopolyhedrovirus (BmNPV) was purified from the digestive juice of B. mori larvae. A homology search of the deduced amino acid sequence of the protein cDNA revealed 56% homology with Drosophila melanogaster lipase and 21% homology with human lipase. As lipase activity of the protein was confirmed in vitro, this protein was desig...
متن کاملCharacterization of a Late Gene, ORF134, from Bombyx mori Nucleopolyhedrovirus
Objective: ORF134 of Bombyx mori nucleopolyhedrovirus (BmNPV) is a homolog of Autographa californica multiple NPV ORF5, but its function is unknown so far. The aim of this study is to characterize BmNPV ORF134 (Bm134). Methods: Transcription, protein expression and subcellular localization of Bm134 in BmN cells and silkworm midguts were analyzed using real-time polymerase chain reaction (RT-PCR...
متن کاملGenome of a Bombyx mori nucleopolyhedrovirus strain isolated from India.
Bombyx mori nucleopolyhedrovirus (BmNPV), a member of the Baculoviridae, is a major pathogen of silkworm and has also been recently developed as an expression vector for heterologous gene expression in the silkworm larvae and pupae. To better understand the diversity of this important baculovirus, we sequenced the complete genome of the BmNPV strain isolated from India, where its host is availa...
متن کاملCharacterization of a late gene, ORF67 from Bombyx mori nucleopolyhedrovirus.
Open reading frame 67 of Bombyx mori nucleopolyhedrovirus (BmORF67) is a homologue of Autographa californica multiple NPV ORF81. The gene is conserved among all baculoviruses and is thus considered a baculovirus core gene. The transcript of BmORF67 was detected at 18-72 h post-infection (p.i.). Polyclonal antiserum raised to a His-BmORF67 fusion protein recognized BmORF67 in infected cell lysat...
متن کاملCharacterization of Bombyx mori nucleopolyhedrovirus Bm17.
Open reading frame17 (Bm17) of Bombyx mori nucleopolyhedrovirus is a highly conserved gene in lepidopteran nucleopolyhedroviruses, suggesting that it performs an important role in the virus life cycle whose function is unknown. In this report, we describe the characterization of Bm17. Reversed transcriptive-PCR (RT-PCR) and Western blot analysis demonstrated that Bm17 was expressed as a late ge...
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ژورنال
عنوان ژورنال: Virology
سال: 2004
ISSN: 0042-6822
DOI: 10.1016/j.virol.2003.12.011